Kev siv Microscope Slides

1. Txoj kev smear yog ib txoj kev ua yeeb yaj kiab uas sib npaug ntawm cov khoom ntawm cov iav swb.

Smear cov ntaub ntawv muaj xws li cov kab mob ib leeg, me me algae, ntshav, kab mob kab mob kua, xoob ntaub so ntswg ntawm tsiaj thiab nroj tsuag, seminal zes, anthers, thiab lwm yam.

Thaum smearing, yuav tsum xyuam xim rau: (1) Lub swb yuav tsum huv si. (2) Cov swb yuav tsum tiaj tus. (3) Cov txheej txheej yuav tsum tsis sib xws. Siv cov tee dej rau sab xis nyob rau hauv nruab nrab ntawm cov swb thiab siv zoo nrog ib tug scalpel hniav los yog toothpick. (4) Lub txheej yuav tsum nyias. Siv lwm tus swb ua ib daim ntawv thawb, maj mam thawb rau ntawm qhov chaw ntawm cov swb dripping nrog cov txheej txheem txheej (lub kaum sab xis ntawm ob lub swb yuav tsum yog 30 degree -45 degree) ntawm sab xis mus rau sab laug, thiab lub tsho ib txheej nyias nyias. . (5) Tsau. Yog tias yuav tsum tau kho, nws tuaj yeem kho nrog cov tshuaj fixatives los yog ziab (cov kab mob). (6) Dyeing. Methylene xiav rau cov kab mob, Reye's staining rau ntshav, thiab qee zaum iodine cawv. Cov tshuaj staining yuav tsum npog tag nrho saum npoo. (7) Yaug. Blot qhuav los yog ci qhuav nrog ntawv absorbent. (8) Foob. Canadian cov pos hniav foob rau lub sijhawm ntev.

2. Cov ntsiav tshuaj nias txoj kev yog ib txoj kev npaj nyob rau hauv uas cov khoom lom yog muab tso rau ntawm lub iav swb thiab daim ntawv npog, thiab ib tug tej yam siab yog siv rau disperse cov ntaub so ntswg hlwb.

3. Txoj kev thauj khoom yog ib txoj hauv kev ua cov qauv swb los ntawm kev sib khi cov khoom siv roj ntsha tag nrho, thiab kev txhim kho ib ntus lossis ruaj khov tuaj yeem ua los ntawm txoj kev no.

Cov khoom thauj khoom muaj xws li: cov kab mob microscopic xws li chlamydomonas algae, daim txhuam cev, amoebas, nematodes; hydra, nplooj epidermis ntawm cov nroj tsuag; Kab tis, taw, qhov ncauj, tib neeg lub qhov ncauj epithelial hlwb, thiab lwm yam.

Kev saib xyuas yuav tsum tau them rau kev tsim cov txheej txheem thauj khoom: (1) Thaum tuav cov swb, nws yuav tsum tau them nyiaj rau flattening lossis muab tso rau ntawm lub platform. Thaum poob dej, tus nqi ntawm cov dej yuav tsum tsim nyog tsuas yog txaus los npog cov npog npog. (2) Cov khoom yuav tsum tau nthuav tawm nrog ib rab koob los yog forceps tsis sib tshooj thiab flattened rau tib lub dav hlau. (3) Thaum muab cov npog npog, maj mam npog cov dej ntws los ntawm ib sab kom tsis txhob muaj npuas. (4) Thaum staining, ib tee dej staining yog muab tso rau ntawm ib sab ntawm daim ntaub npog thiab nyiam los ntawm lwm sab nrog cov ntaub ntawv absorbent kom sib npaug xim cov qauv hauv qab daim npog. Tom qab cov xim, siv tib txoj kev, tee ib tee dej, nqus tawm cov staining tov, thiab saib nws nyob rau hauv ib lub microscope.

4. Slice yog ib daim ntawv swb uas ua los ntawm cov hlais nyias txiav los ntawm cov khoom muaj sia.

Raws li qhov yuav tsum tau ua, cov seem pub dawb tuaj yeem ua nrog cov hniav, lossis cov ntaub so ntswg tuaj yeem muab tso rau hauv paraffin lossis collodion lossis khov ntawm qhov kub thiab txias thiab hlais nrog microtome. Txiav rau hauv 5 ~ 10 micron nplooj ntawv rau kev soj ntsuam lub teeb pom kev zoo. Ultrathin ntu txiav nrog epoxy resin los yog methacrylic acid embedded ntaub so ntswg blocks, nrog ib tug tuab ntawm 20 ~ 50 nanometers, tshwj xeeb tshaj yog tsim los rau kev soj ntsuam nyob rau hauv electron microscopes. Cov ntu siv rau kev qhia dav dav, xws li cov lus qhia hauv paus thiab stems, feem ntau hu ua paraffin seem.